Dental and Medical Problems

Dent. Med. Probl.
Index Copernicus (ICV) – 109.28, MNiSW – 11
Rejection rate – 43.33%
License – Creative Commons: Attribution 3.0 Unported (CC BY 3.0)
ISSN 1644-387X (print),   ISSN 2300-9020 (online)
Periodicity – quarterly

Download PDF

Dental and Medical Problems

2018, vol. 55, nr 4, October-December, p. 359–366

doi: 10.17219/dmp/96290

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Creative Commons BY-NC-ND 3.0 Open Access

Comparative evaluation of the early effects of the low-level laser therapy versus intra-articular steroids on temporomandibular joint acute osteoarthritis in rats: A histochemical, molecular and imaging evaluation

Ocena porównawcza wczesnej reakcji na laser małej mocy w odniesieniu do dostawowego podawania steroidów w ostrym zapaleniu stawów skroniowo-żuchwowych u szczurów – badanie histochemiczne, molekularne i obrazowe

Nermeen Abubakr1,A,B,C,D,E,F, Zeinab Salem1,B,C,D,E,F, Zoba Ali1,A,B,D,E,F, Manal El Assaly1,A,C,D,E,F

1 Department of Oral Biology, Faculty of Dentistry, Cairo University, Egypt

Abstract

Background. Osteoarthritis (OA) is a serious degenerative joint disease. It is one of the main causes of disability in the world. Current treatment modalities have numerous side effects. Subsequently, health experts are looking for alternative therapies.
Objectives. The aim of the study was to evaluate the early effects of low-level laser therapy (LLLT) vs intraarticular (IA) corticosteroids (CS) on acute temporomandibular joint osteoarthritis (TMJOA).
Material and Methods. Sixty rats were divided into 3 groups: group 1– untreated OA; group 2 – OA treated with CS; and group 3 – OA treated with LLL. Half of the animals in each group were sacrificed at 1 and 4 weeks post treatment. The temporomandibular joint was dissected and evaluated histochemically, using quantitative real-time polymerase chain reaction (qRT-PCR), and radiographically.
Results. Histochemically, Safranin-O staining revealed an obvious reduction in proteoglycans in the untreated osteoarthritic group. However, both of the treated groups showed a moderate increase in glycosaminoglycan (GAG) staining. As for the qRT-PCR results, caspase-3 showed the highest mean value in the untreated OA group, followed by the CS group, while the lowest mean value was recorded in the LLL group. Radiographically, the condyle showed erosion, flattening, osteophyte formation, and sclerosis in the untreated group, but there was great improvement in both of the treated groups.
Conclusion. Both laser and cortisone showed reparative and formative effects, as evidenced by the increases in the proteoglycan content. However, LLL was superior in its anti-apoptotic effects. Cone beam computed tomography (CBCT) is a valuable tool in assessing osseous abnormalities.

Key words

osteoarthritis, temporomandibular joint, glucocorticoids, low-level laser therapy, caspase-3

Słowa kluczowe

zapalenie stawów, staw skroniowo-żuchwowy, glikokortykosteroidy, terapia laserem małej mocy, kaspaza-3

References (30)

  1. Kim JH, Ok SM, Heo JY, et al. The clinical and radiographic features of patients with temporomandibular joint osteoarthritis: Comparison of adolescents and middle-old aged Koreans. J Oral Med Pain. 2014;39:2–9.
  2. Breedveld FC. Osteoarthritis – the impact of a serious disease. Rheumatol. 2004;43(Suppl 1):i4–8.
  3. Galanti C, Musumeci G, Valentino J, Giunta S, Castorina S. A role for apoptosis in temporomandibular joint disc degeneration. A contemporary review. Ital J Anat Embryol. 2013;118:151–158.
  4. Cho BH, Jung YH. Intra- and interobserver agreement of computed tomography in assessment of the mandibular condyle. Korean J Oral Maxillofac Radiol. 2007;37:191–195.
  5. Clark AR, Belvisi MG. Maps and legends: The quest for dissociated ligands of the glucocorticoid receptor. Pharmacol Ther. 2012;134:54–67.
  6. Stephens MB, Beutler AI, O’Connor FG. Musculoskeletal injections: A review of the evidence. Am Fam Physician. 2008;78:971–976.
  7. Mountziaris PM, Kramer PR, Mikos AG. Emerging intra-articular drug delivery systems for the temporomandibular joint. Methods. 2009;47:134–140.
  8. Huang Z, Ma J, Chen J, Shen B, Pei F, Kraus VB. The effectiveness of low-level laser therapy for nonspecific chronic low back pain: A systematic review and meta-analysis. Arthritis Res Ther. 2015;17:360.
  9. Herman JH, Khosla RC. In vitro effects of Nd:YAG laser radiation on cartilage metabolism. J Rheumatol. 1988;15:1818–1826.
  10. Wang XD, Kou XX, He DQ, et al. Progression of cartilage degradation, bone resorption and pain in rat temporomandibular joint osteoarthritis induced by injection of iodoacetate. PLoS ONE. 2012;7:e45036.
  11. Connolly KM, Stecher VJ, Pruden DJ. Effect of auranofin on plasma fibronectin, C reactive protein, and albumin levels in arthritic rats. Ann Rheum Dis. 1988;47:515–521.
  12. El-Hakim IE, Abdel-Hamid IS, Bader A. Temporomandibular joint (TMJ) response to intra-articular dexamethasone injection following mechanical arthropathy: A histological study in rats. Int J Oral Maxillofac Surg. 2005;34:305–310.
  13. Khozeimeh F, Moghareabed A, Allameh M, Baradaran S. Comparative evaluation of low-level laser and systemic steroid therapy in adjuvant-enhanced arthritis of rat temporomandibular joint: A histological study. Dent Res J. 2015;12:215–223.
  14. Mazzetto MO, Carrasco TG, Bidinelo EF, Pizzo RC, Mazzetto RG. Low intensity laser application in temporomandibular disorders: A phase I double-blind study. Cranio. 2007;25:186-192.
  15. Steven L, Wendy U, Raymond A, et al. AVMA Guidelines for the Euthanasia of Animals: 2013 Edition. Schaumburg (IL): American Veterinary Medical Association; 2013.
  16. Lampropoulou-Adamidou K, Lelovas P, Karadimas EV, et al. Useful animal models for the research of osteoarthritis. Europ J Orthop Surg Traumatol. 2014;24:263–271.
  17. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: An animal model of osteoarthritis. Toxicol Pathol. 2003;31:138–139.
  18. Clark AR. MAP kinase phosphatase 1: A novel mediator of biological effects of glucocorticoids? J Endocrinol. 2003;178:5–12.
  19. Karu TI, Kolyakov SF. Exact action spectra for cellular responses relevant to phototherapy. Photomed Laser Surg. 2005;23:355–361.
  20. Flahiff CM, Narmoneva DA, Huebner JL, Kraus VB, Guilak F, Setton LA. Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage. J Biomech. 2002;35:1285–1290.
  21. Lu YC, Evans CH, Grodzinsky AJ. Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokines. Arthritis Res Ther. 2011;13:R142.
  22. Gottlieb T, Jörgensen B, Rohde E, Müller G, Scheller EE. The influence of irradiation with low-level diode laser on the proteoglycan content in arthritically changed cartilage in rabbits. Med Laser Applicat. 2006;21:53–59.
  23. Kourí-Flores JB, Abbud-Lozoya KA, Roja-Morales L. Kinetics of the ultrastructural changes in apoptotic chondrocytes from an osteoarthrosis rat model: A window of comparison to the cellular mechanism of apoptosis in human chondrocytes. Ultrastruct Pathol. 2002;26:33–40.
  24. Chagin AS, Karimian E, Zaman F, Takigawa M, Chrysis D, Sävendahl L. Tamoxifen induces permanent growth arrest through selective induction of apoptosis in growth plate chondrocytes in cultured rat metatarsal bones. Bone. 2007;40:1415–1424.
  25. Lee JH, Oh SH, Kim TH, Go YY, Song JJ. Anti-apoptotic effect of dexamethasone in an ototoxicity model. Biomater Res. 2017;21:4.
  26. Lin HD, He CQ, Luo QL, Zhang JL, Zeng DX. The effect of low-level laser to apoptosis of chondrocyte and caspases expression, including caspase-8 and caspase-3 in rabbit surgery-induced model of knee osteoarthritis. Rheumatol Int. 2012;32:759–766.
  27. Westesson PL. Reliability and validity of imaging diagnosis of temporomandibular joint disorder. Adv Dent Res. 1993;7:137–151.
  28. Mani FM, Sivasubramanian SS. A study of temporomandibular joint osteoarthritis using computed tomographic imaging. Biomed J. 2016;39:201–206.
  29. Ringold S, Torgerson TR, Egbert MA, Wallace CA. Intraarticular corticosteroid injections of the temporomandibular joint in juvenile idiopathic arthritis. J Rheumatol. 2008;35:1157–1164.
  30. Mazzetto MO, Hotta TH, Pizzo RC. Measurements of jaw movements and TMJ pain intensity in patients treated with GaAlAs laser. Braz Dent J. 2010;21:356–360.