Dental and Medical Problems
2018, vol. 55, nr 4, October-December, p. 379–382
Publication type: original article
Remineralization of artificial carious lesions using a novel fluoride incorporated bioactive glass dentifrice
Remineralizacja preparowanych in vitro ubytków próchnicowych środkiem zawierającym nowe bioaktywne szkło wzbogacone fluorkami
1 Dental Intern, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
2 Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
Background. Remineralization potential of dentifrices with novel compositions that can restore minerals back into incipient carious lesions has not been extensively studied so far.
Objectives. The aim of this study was to assess the efficacy of a dentifrice based on novel fluoride incorporated bioactive glass in remineralizing artificial carious lesions in human enamel, and compare it with a standard fluoride-containing dentifrice.
Material and Methods. Twenty-four human extracted teeth were sectioned at the cementoenamel junction to obtain enamel blocks. These blocks (n = 24) were randomly divided into 3 groups, with each group containing 8 specimens: group 1 (negative control group; distilled water), group 2 (positive control group; fluoride toothpaste) and group 3 (test group; BioMinTM F toothpaste). Artificial carious lesions were produced in the enamel surfaces by exposing them to a demineralization solution (6% citric acid, pH 2.2) for 96 h. After demineralization, the specimens were brushed with manual toothbrushes in a toothbrush simulation machine (each sample received 800 strokes). For brushing the specimens from group 1, 20 mL of distilled water was used, for group 2 – 20 mL of slurry of toothpaste mixed with artificial saliva, and for group 3 – 20 mL of slurry of toothpaste (BioMin F) mixed with artificial saliva. The micro-hardness data (VHN – Vickers hardness number) was collected at baseline (sound enamel), post-demineralization and post-remineralization.
Results. The biggest difference between the post-remineralization and post-demineralization values was observed in group 3 (mean VHN = 118.73), followed by group 2 (mean VHN = 60.54) and group 1 (mean VHN = 47.44). All the groups revealed significant differences (p < 0.05) when the post-demineralization and post-remineralization values were compared to baseline values within each group.
Conclusion. The BioMin F group outperformed the other 2 groups in terms of remineralizing the demineralized enamel structure.
enamel, remineralization, fluoride bioactive glass
szkliwo, remineralizacja, bioaktywne szkło wzbogacone fluorkami
- Featherstone JD. Dental caries: A dynamic process. Aust Dent J. 2008;53:286–291.
- Kawasaki K, Ruben J, Tsuda H, Huysmans MC, Takagi O. Relationship between mineral distributions in dentine lesions and subsequent remineralization in vitro. Caries Res. 2000;34:395–403.
- González-Cabezas C. The chemistry of caries: Remineralization and demineralization events with direct clinical relevance. Dent Clin North Am. 2010;54:469–478.
- Collins WJN, Walsh TF, Figures KH. A Handbook for Dental Hygienists. 4th ed. Oxford, UK: Butterworth-Heinemann. 1998:272–273.
- Jones JR. Review of bioactive glass: From Hench to hybrids. Acta Biomater. 2013;9:4457–4486.
- Krishnan V, Lakshmi T. Bioglass: A novel biocompatible innovation. J Adv Pharm Technol Res. 2013;4:78–83.
- Farooq I, Imran Z, Farooq U, Leghari A, Ali H. Bioactive glass: A material for the future. World J Dent. 2012;3:199–201.
- Pradeep K, Rao PK. Remineralizing agents in the non-invasive treatment of early carious lesions. Int J Dent Case Rep. 2011;1:73–84.
- Mohammed RN, Lynch RJ, Anderson P. Effects of fluoride concentration on enamel demineralization kinetics in vitro. J Dent. 2014;42:613–618.
- https://www.biomin.co.uk/products/biomintm-f-toothpaste. Accessed May 10, 2018.
- Fusayama T, Katayori T, Nomoto S. Corrosion of gold and amalgam placed in contact with each other. J Dent Res. 1963;42:1183–1197.
- Wang Y, Mei L, Gong L, et al. Remineralization of early enamel caries lesions using different bioactive elements containing toothpastes: An in vitro study. Technol Health Care. 2016;24:701–711.
- Somaraj V, Shenoy RP, Shenoy Panchmal G, Kumar V, Jodalli PS, Sonde L. Effect of herbal and fluoride mouth rinses on Streptococcus mutans and dental caries among 12–15-year-old school children: A randomized controlled trial. Int J Dent. 2017;2017:5654373.
- Clark MB, Slayton RL; Section on Oral Health. Fluoride use in caries prevention in the primary care setting. Pediatrics. 2014;134:626–633.
- Khalid MD, Khurshid Z, Zafar MS, Farooq I, Khan RS, Najmi A. Bioactive glasses and their applications in dentistry. J Pak Dent Assoc. 2017;26:32–38.
- Aasenden R, Brudevold F, Richardson B. Clearance of fluoride from the mouth after topical treatment or the use of a fluoride mouthrinse. Arch Oral Biol. 1968;13:625–636.
- Brauer DS, Karpukhina N, O’Donnell MD, Law RV, Hill RG. Fluoride-containing bioactive glasses: Effect of glass design and structure on degradation, pH and apatite formation in simulated body fluid. Acta Biomater. 2010;6:3275–3282.
- Shaikh K, Pereira R, Gillam DG, Phad S. Comparative evaluation of desensitizing dentifrices containing BioMin®, Novamin® and fluoride on dentinal tubule occlusion before and after a citric acid challenge – a scanning electron microscope in-vitro study. J Odontol. 2018;2:1000105.
- ten Cate JM. Current concepts on the theories of the mechanism of action of fluoride. Acta Odontol Scand. 1999;57:325–329.
- Farooq I, Tylkowski M, Müller S, Janicki T, Brauer DS, Hill RG. Influence of sodium content on the properties of bioactive glasses for use in air abrasion. Biomed Mater. 2013;8:065008.