Dental and Medical Problems

Dent. Med. Probl.
Index Copernicus (ICV 2018) – 113.05
MNiSW – 20
Average rejection rate – 70.86%
ISSN 1644-387X (print)
ISSN 2300-9020 (online)
Periodicity – quarterly

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Dental and Medical Problems

2020, vol. 57, nr 2, April-June, p. 137–144

doi: 10.17219/dmp/117721

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Novel method of determining the periodontal regenerative capacity of T‑PRF and L‑PRF: An immunohistochemical study

Nowa metoda określania periodontologicznego potencjału regeneracyjnego T‑PRF i L‑PRF – badanie immunohistochemiczne

Hirak Shubhra Bhattacharya1,D,E,F, Shiva Shankar Gummaluri1,A,B,C,D,E,F, Madhusudan Astekar2,C,D,E,F, Ram Kumar Gummaluri3,A,B,C

1 Department of Periodontology and Implantology, Institute of Dental Sciences, Bareilly, India

2 Department of Oral and Maxillofacial Pathology and Microbiology, Institute of Dental Sciences, Bareilly, India

3 Department of Chemistry, MVGR College of Engineering, Chintalavalasa, India

Abstract

Background. Platelet-rich fibrin (PRF) plays an important role in the regeneration of the lost periodontal tissues. Immunohistochemistry (IHC) is the most sensitive staining technique for the identification and localization of specific cells. There are few studies in the available literature which use IHC to compare PRF prepared from titanium and silica glass tubes.
Objectives. The aim of the study was to use IHC to evaluate and compare cells present in the PRF membrane prepared from titanium and silica glass tubes.
Material and Methods. Blood was drawn from 10 healthy volunteers and PRF was prepared from titanium and silica glass tubes. Immunohistochemical staining for the localization, distribution and pattern of cells present in PRF with the CD 3, CD 15, CD 20, CD 34, CD 61, and CD 163 antibodies was carried out. A statistical analysis including the χ2 test, independent t-test and unpaired t-test was performed to determine significant differences.
Results. There were significantly more T cells, B‑lymphocytes and platelets, with a strongly positive staining in terms of the cell distribution and the labeling index in the T-PRF group in comparison with the L-PRF group. However, in terms of localization, a stronger positive staining was obtained with platelets in the T-PRF group and stem cells in the L-PRF group. In terms of the cell pattern, a significantly stronger positive staining was obtained by neutrophils in the L-PRF group and B‑lymphocytes in the T‑PRF group.
Conclusion. Titanium PRF has the edge over PRF prepared from silica glass tubes, and emerged as a better alternative for use in the field of periodontal regeneration.

Key words

immunohistochemistry, platelet-rich fibrin, periodontal regeneration, histological techniques

Słowa kluczowe

immunohistochemia, fibryna bogatopłytkowa, regeneracja periodontologiczna, techniki histologiczne

References (39)

  1. Chen FM, Jin Y. Periodontal tissue engineering and regeneration: Current approaches and expanding opportunities. Tissue Eng Part B Rev. 2010;16(2):219–255.
  2. Dzobo K, Thomford NE, Senthebane DA, et al. Advances in regene­rative medicine and tissue engineering: Innovation and transformation of medicine. Stem Cells Int. 2018;2018:2495848.
  3. Agrawal AA. Evolution, current status and advances in application of platelet concentrate in periodontics and implantology. World J Clin Cases. 2017;5(5):159–171.
  4. Choukroun J, Adda F, Schoeffer C, Vervelle A. PRF: An opportunity in perio-implantology [in French]. Implantodontie. 2000;42:55–62.
  5. Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e37–e44.
  6. Agarwal A, Gupta ND, Jain A. Platelet-rich fibrin combined with decalcified freeze-dried bone allograft for the treatment of human intrabony periodontal defects: A randomized split mouth clinical trail. Acta Odontol Scand. 2016;74(1):36–43.
  7. Pradeep AR, Nagpal K, Karvekar S, Patnaik K, Naik SB, Guruprasad CN. Platelet‐rich fibrin with 1% metformin for the treatment of intrabony defects in chronic periodontitis: A randomized controlled clinical trial. J Periodontol. 2015;86(6):729–737.
  8. Verma UP, Yadav RK, Dixit M, Gupta A. Platelet-rich fibrin: A paradigm in periodontal therapy – a systematic review. J Int Soc Prev Community Dent. 2017;7(5):227–233.
  9. Ghanaati S, Booms P, Orlowska A, et al. Advanced platelet-rich fibrin: A new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol. 2014;40(6):679–689.
  10. Dohan Ehrenfest DM, de Peppo GM, Doglioli P, Sammartino G. Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): A gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors. 2009;27(1):63–69.
  11. O’Connell SM. Safety issues associated with platelet-rich fibrin method. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103(5):587–593.
  12. Tunalı M, Özdemir H, Küçükodacı Z, Akman S, Fıratlı E. In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): A new platelet concentrate. Brit J Oral Maxillofac Surg. 2013;51(5):438–443.
  13. Tunalı M, Özdemir H, Küçükodacı Z, et al. A novel platelet concentrate for guided bone regeneration: Titanium-prepared platelet-rich fibrin. Biomed Res Int. 2014;2014:209548.
  14. Duan X, Lin Z, Lin X, et al. Study of platelet‐rich fibrin combined with rat periodontal ligament stem cells in periodontal tissue regeneration. J Cell Mol Med. 2018;22(2):1047–1055.
  15. Di Liddo R, Bertalot T, Borean A, et al. Leucocyte and platelet‐rich fibrin: A carrier of autologous multipotent cells for regenerative medicine. J Cell Mol Med. 2018;22(3):1840–1854.
  16. He L, Lin Y, Hu X, Zhang Y, Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108(5):707–713.
  17. Huang FM, Yang SF, Zhao JH, Chang YC. Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells. J Endod. 2010;36(10):1628–1632.
  18. Mazor Z, Horowitz RA, Del Corso M, Prasad HS, Rohrer MD, Dohan Ehrenfest DM. Sinus floor augmentation with simultaneous implant placement using Choukroun’s platelet‐rich fibrin as the sole grafting material: A radiologic and histologic study at 6 months. J Periodontol. 2009;80(12):2056–2064.
  19. Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M. Applications of immunohistochemistry. J Pharm Bioallied Sci. 2012;4(Suppl 2): S307–S309.
  20. Bancroft JD, Cook H, Turner D. Manual of Histological Techniques and Their Diagnostic Application. 3rd ed. Philadelphia, USA: Churchill Livingstone/Elsevier Health Sciences; 1996:986–998.
  21. Alrani D, Niranjan KC, Sarathy NA. Assessment of proliferative index between the tumor margin, center of tumor, and the invasive tumor front of oral squamous cell carcinoma with the help of Mcm-2: An immunohistochemical study. Appl Immunohistochem Mol Morphol. 2020;28(1):30–35.
  22. Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e56–e60.
  23. Arora S, Agnihotri N. Platelet derived biomaterials for therapeutic use: Review of technical aspects. Indian J Hematol Blood Transfus. 2017;33(2):159–167.
  24. Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: A review of biology and applications in plastic surgery. Plast Reconstr Surg. 2006;118(6):147e–159e.
  25. Jenne CN, Urrutia R, Kubes P. Platelets: Bridging hemostasis, inflammation, and immunity. Int J Lab Hematol. 2013;35(3):254–261.
  26. Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: The first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg. 2018;44(1):87–95.
  27. Soltan M, Rohrer MD, Prasad HS. Monocytes: Super cells for bone regeneration. Implant Dent. 2012;21(1):13–20.
  28. Tan KW, Chong SZ, Wong FH, et al. Neutrophils contribute to inflammatory lymphangiogenesis by increasing VEGF-A bioavailability and secreting VEGF-D. Blood. 2013;122(22):3666–3677.
  29. Ekström K, Omar O, Granéli C, Wang X, Vazirisani F, Thomsen P. Monocyte exosomes stimulate the osteogenic gene expression of mesenchymal stem cells. PloS One. 2013;8(9):e75227.
  30. Maciel J, Oliveira MI, Colton E, et al. Adsorbed fibrinogen enhances production of bone- and angiogenic-related factors by monocytes/macrophages. Tissue Eng Part A. 2014;20(1–2):250–263.
  31. Sîrbulescu RF, Boehm CK, Soon E, et al. Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions. Wound Repair Regen. 2017;25(5):774–791.
  32. Boyce DE, Jones WD, Ruge F, Harding KG, Moore K. The role of lymphocytes in human dermal wound healing. Br J Dermatol. 2000;143(1):59–65.
  33. Chatterjee A, Debnath K, Ali MM, Babu C, Gowda PL. Comparative histologic evaluation of titanium platelet-rich fibrin and platelet-rich fibrin in hypertensive and smoker participants: A cell cytology study. J Indian Soc Periodontol. 2017;21(3):195–200.
  34. Mitra DK, Potdar PN, Prithyani SS, Rodrigues SV, Shetty GP, Talati MA. Comparative study using autologous platelet-rich fibrin and titanium prepared platelet-rich fibrin in the treatment of infrabony defects: An in vitro and in vivo study. J Indian Soc Periodontol. 2019;23(6):554–561.
  35. Yajamanya SR, Chatterjee A, Babu CN, Karunanithi D. Fibrin network pattern changes of platelet-rich fibrin in young versus old age group of individuals: A cell block cytology study. J Indian Soc Periodontol. 2016;20(2):151–156.
  36. Ogawa M, LaRue AC, Mehrotra M. Hematopoietic stem cells are pluripotent and not just “hematopoietic”. Blood Cells Mol Dis. 2013;51(1):3–8.
  37. Lim WF, Inoue-Yokoo T, Tan KS, Lai MI, Sugiyama D. Hematopoietic cell differentiation from embryonic and induced pluripotent stem cells. Stem Cell Res Ther. 2013;4(3):71–82.
  38. Nicolaidou V, Wong MM, Redpath AN, et al. Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation. PloS One. 2012;7(7):e39871.
  39. Wang L, Zhao Y, Shi S. Interplay between mesenchymal stem cells and lymphocytes: Implications for immunotherapy and tissue regeneration. J Dent Res. 2012;91(11):1003–1010.